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![BAFF Antibody [Buffy-2]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/1280x1280/products/552807/760381/porsci_lo__79508.1648973713__05117.1649086075.png?c=2 "BAFF Antibody [Buffy-2]")
![BAFF Antibody [Buffy-2]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/100x100/products/552807/760381/porsci_lo__79508.1648973713__05117.1649086075.png?c=2 "BAFF Antibody [Buffy-2]")
Product Description
BAFF Antibody [Buffy-2] | XA-1012 | ProSci
Host: Rat
Reactivity: Human, Mouse
Homology: N/A
Immunogen: BAFF (monoclonal Buffy-2) antibody was raised against recombinant human soluble BAFF (aa. 83-285) .
Research Area: Chemokines & Cytokines, Cancer
Tested Application: WB, IHC, Flow
Application: BAFF antibody can be used to recognize membrane-bound and soluble human and mouse (weak) BAFF by Flow Cytometry, Immunohistochemistry (frozen sections, paraffin sections) and Western Blot. Method: HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of full length hBAFF (thick line) . Cells were incubated on ice for 30min in 50 μl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1 μg/mL of MAb to BAFF (Buffy-2) (FITC) . After washing, cells were analyzed by flow cytometry. Method: Tissues are in paraffin sections. Sections are de-waxed and rehydrated, then cooked. Sections are stained in wet boxes, rehydrated and finally prepared as coverslip slides using Eukitt solution. Please inquire for complete protocol..
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Anti BAFF (monoclonal Buffy-2) antibody was purified to ≥95% in SDS-PAGE.
Clonality: Monoclonal
Clone: Buffy-2
Isotype: IgM
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS containing 0.02% sodium azide
Concentration: N/A
Storage Condition: BAFF antibody can be stored at 4˚C, stable for one year.
Alternate Name: N/A
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: N/A
![BAFF Antibody [Buffy-2]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/552807/760381/porsci_lo__79508.1648973713__05117.1649086075.png?c=2 "BAFF Antibody [Buffy-2]")
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![BAFF Antibody [Buffy-1] (FITC)](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/552806/760379/porsci_lo__79508.1648973713__88925.1649086075.png?c=2 "BAFF Antibody [Buffy-1] (FITC)")
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