Product Description
CD4 Antibody [RM4-5] (Violet-450) | 76-371 | ProSci
Host: Rat
Reactivity: Mouse
Homology: N/A
Immunogen: N/A
Research Area: Immunology
Tested Application: Flow
Application: N/A
Specificiy: The RM4-5 monoclonal antibody specifically reacts with mouse CD4, also known as L3T4, a 55 kDa differentiation antigen expressed by the majority of thymocytes, subpopulations of mature T cells (like major histocompatibility complex class II-restricted T lymphocytes) , a subset of natural killer T cells, and on pluripotent hematopoietic stem cells.
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Clonality: Monoclonal
Clone: RM4-5
Isotype: Rat IgG2a, kappa
Conjugate: Violet-450
Physical State: liquid
Buffer: Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
Concentration: batch dependent
Storage Condition: The product should be stored undiluted at 4˚C and should be protected from prolonged exposure to light. Do not freeze.
Alternate Name: L3T4, Ly-4, Cd4
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: The RM4-5 monoclonal antibody specifically reacts with mouse CD4, also known as L3T4, a 55 kDa differentiation antigen expressed by the majority of thymocytes, subpopulations of mature T cells (like major histocompatibility complex class II-restricted T lymphocytes) , a subset of natural killer T cells, and on pluripotent hematopoietic stem cells. CD4 binds to the major histocompatibility complex class II (MHC class II) and enhances T lymphocyte development and mature T cells functions. In T lymphocytes, CD4 binds to the cytoplasmic tail of enzyme tyrosine kinase (p56lck) .Binding of RM4-5 is blocked by the anti-mouse CD4 clone GK1.5.BG Violet 450 conjugate is an alternative to the Pacific Blue, eFluor 450, or BD Horizon V450 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 450nm.