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![FasL Antibody [A11]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/1280x1280/products/552798/760365/porsci_lo__79508.1648973713__97908.1649086073.png?c=2 "FasL Antibody [A11]")
![FasL Antibody [A11]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/100x100/products/552798/760365/porsci_lo__79508.1648973713__97908.1649086073.png?c=2 "FasL Antibody [A11]")
Product Description
FasL Antibody [A11] | XA-1000 | ProSci
Host: Rat
Reactivity: Mouse
Homology: N/A
Immunogen: FasL (monoclonal A11) antibody was raised against a synthetic peptide corresponding to amino acids 196-220 of mouse FasL.
Research Area: Apoptosis
Tested Application: IHC, Flow
Application: FasL antibody can be used for the detection of mouse FasL by flow cytometry and immunohistochemistry (frozen sections and paraffin sections) .Method: Cryostat sections (6-8 μm) were air dried, fixed in 4% paraformaldehyde in PBS and used immediately. Sections were incubated for 1 hr at room temperature (RT) with 20 μg/mL of A11 antibody in PBS, 0.2% BSA, 0.05% Tween 20. After rinsing in PBS, a FITC-conjugated anti-rat IgM was added for 30 min., slides washed again in PBS and visualized using a fluorescence microscope.Paraffin sections: Dewax in xylene (2x10 min) , EtOH 100% (2x10 min) , block endogeneous peroxidase (0.6% H2O2 in Methanol 10 min) , rehydrate through graded EtOH (100%, 95%, 70%) , rinse in H20, unmask epitopes by heating slides in citrate buffer (0.01M, pH 6.0) 2x5 min in microwave oven (650-750W) , cool down to RT 20 min, wash 1x in PBS, incubate with A11 20 μg/mL antibody, wash in PBS, incubate in anti-rat IgM HRP, wash in PBS, develop in appropriate substrate solution (DAB or AEC) .
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: FasL (monoclonal A11) antibody was purified to ≥95% in SDS-PAGE.
Clonality: Monoclonal
Clone: A11
Isotype: IgM
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS containing 0.02% sodium azide
Concentration: N/A
Storage Condition: FasL antibody can be stored at 4˚C, stable for one year.
Alternate Name: N/A
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: N/A
![FasL Antibody [A11]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/552798/760365/porsci_lo__79508.1648973713__97908.1649086073.png?c=2 "FasL Antibody [A11]")
![FasL Antibody [Mike-2]](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/552803/760375/porsci_lo__79508.1648973713__39068.1649086074.png?c=2 "FasL Antibody [Mike-2]")
