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Product Description
Dynamin (phospho Ser774) Antibody | XPS-1006 | ProSci
Host: Sheep
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: Dynamin (Ser774) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser774 of human, mouse and rat dynamin.
Research Area: Neuroscience, Phospho-Specific
Tested Application: WB, IF, IHC
Application: Dynamin antibody for phospho-Ser774 dynamin labels the purified protein phosphorylated in vitro by Cdk5 but not PKC (Tan, et al. 2003) . The labeling of the dynamin band in Western blots of rat brain was blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Sheep anti- Dynamin (Ser774) does not cross react with other purified substrates of CDK5 (e.g. amphiphysin and synapsin) . Applications include Immunoblotting (IB) , Immunofluorescence (IF) , Immunohistochemistry (IHC) , Western Blots (WB) . Human, mouse and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for IB and WB.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: 95
Validation: N/A
Isoform: N/A
Purification: Affinity Purified
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: N/A
Concentration: N/A
Storage Condition: For long term storage -80˚C is recommended, but shorter term storage at -20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20˚C.
Alternate Name: N/A
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis. Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser774 and Ser778 that are the phosphorylation sites on dynamin phosphorylated in vivo. Phosphorylation of these sites on dynamin is thought to play a key role in synaptic vesicle trafficking.
