Product Description
CBX4 Antibody | 7007 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: CBX4 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human CBX4.
The immunogen is located within amino acids 50 - 100 of CBX4.
Research Area: Stem Cell
Tested Application: E, WB, IF
Application: CBX4 antibody can be used for detection of CBX4 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: Two alternatively spliced transcript variants have been observed.
Positive Control 1: Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 2: Cat. No. 10-301 - Human Brain Tissue Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: 62 kDa
Validation: N/A
Isoform: N/A
Purification: CBX4 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: CBX4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: CBX4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: CBX4 Antibody: PC2, NBP16, E3 SUMO-protein ligase CBX4, Chromobox protein homolog 4, Pc2
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: CBX4 Antibody: Polycomb group (PcG) proteins are chromatin-associated proteins which are important for embryonic and adult stem cell self-renewal and maintenance. At least two distinct human PcG complexes have been identified. Polycomb repressive complex I (PRC1) contains CBX4/Pc2, HPH1 and RING domain-containing proteins (RING1, RING2 and BMI1) . CBX4 (Chromobox protein homolog 4 or PC2) , which functions as a transcriptional suppressor, is a candidate of KyoT2-binding proteins. It is a E3 SUMO-protein ligase which facilitates SUMO1 conjugation. Depletion of CBX4 results in decreased cellular resistance to ionizing radiation. CBX4 is directly involved in the cellular response to DNA damage.