Product Description
CLPH Antibody | 5497 | ProSci
Host: Rabbit
Reactivity: Human, Rat
Homology: Predicted species reactivity based on immunogen sequence: Bovine: (75%) , Mouse: (70%)
Immunogen: CLPH antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human CLPH.
The immunogen is located within the last 50 amino acids of CLPH.
Research Area: Stem Cell
Tested Application: E, WB, IHC-P
Application: CLPH antibody can be used for detection of CLPH by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Specificiy: N/A
Positive Control 1: Cat. No. 1313 - Human Testis Tissue Lysate
Positive Control 2: Cat. No. 11-701 - Human Testis Tissue Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: CLPH Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: CLPH Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: CLPH antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: CLPH Antibody: CLPH, C4orf35, NYD-SP26, Calcium-binding and spermatid-specific protein 1, Testis development protein NYD-SP26
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: CLPH Antibody: CLPH (Casein-Like phosphoprotein) is a recently discovered, evolutionarily conserved, testis-specific cytoplasmic protein expressed exclusively in postmeiotic germ cells in rat spermatogenesis. CLPH is a novel casein kinase 2-phosphorylated disordered and calcium-binding protein that displays strong variation in relative expression levels during spermatogenesis. It is frequently associated with the mitochondrial inner membrane during the last steps of spermatid differentiation and is involved in the extremely complex structural rearrangements occurring in haploid germ cells during spermiogenesis.