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Product Description
RBM35B Antibody | 6187 | ProSci
Host: Rabbit
Reactivity: Human, Mouse
Homology: Predicted species reactivity based on immunogen sequence: Rat: (80%)
Immunogen: RBM35B antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human RBM35B.
The immunogen is located within the last 50 amino acids of RBM35B.
Research Area: Stem Cell
Tested Application: E, WB, IHC-P, IF
Application: RBM35B antibody can be used for detection of RBM35B by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: RBM35B antibody is predicted to not cross-react with other RBM35/ESRP family members. At least two isoforms of RBM35B are known to exist; this antibody will detect both isoforms.
Positive Control 1: Cat. No. 1302 - Human Lung Tissue Lysate
Positive Control 2: Cat. No. 10-101 - Human Lung Tissue Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: RBM35B Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: RBM35B Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: RBM35B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: RBM35B Antibody: RBM35B, RBM35B, PP7059, Epithelial splicing regulatory protein 2, RNA-binding motif protein 35B
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: RBM35B Antibody: RBM35A, also known as ESRP2, is a mRNA splicing factor that with its related protein RBM35A (ESRP1) are coordinators of an epithelial cell-type-specific splicing program. Both RBM35B and RBM35A are involved in posttranscriptional regulation of a number of genes such as FGFR2, CD44, CTNND1, and ENAH by exerting a differential effect on protein translation via 5' UTRs of mRNAs, suggesting that these proteins are global regulators of an epithelial regulatory network. Loss of this global ESRP-regulated epithelial splicing programme induces the phenotypic changes in cell morphology that are observed during the epithelial-mesenchymal transition.
