Product Description
TMIGD1 Antibody | 7615 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: Predicted species reactivity based on immunogen sequence: Bovine: (72%)
Immunogen: TMIGD1 antibody was raised against an 18 amino acid peptide near the center of human TMIGD1.
The immunogen is located within amino acids 150 - 200 of TMIGD1.
Research Area: Stem Cell
Tested Application: E, WB, IHC-P, IF
Application: TMIGD1 antibody can be used for detection of TMIGD1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificiy: TMIGD1 antibody is human, mouse and rat reactive. At least two isoforms of TMIGD1 are known to exist; this antibody will detect both isoforms.
Positive Control 1: Cat. No. 1464 - Rat Liver Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 29 kDa
Observed: 24 kDa
Validation: N/A
Isoform: N/A
Purification: TMIGD1 antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: TMIGD1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: TMIGD1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: TMIGD1 Antibody: TMIGD, UNQ9372, TMIGD, UNQ9372/PRO34164, Transmembrane and immunoglobulin domain-containing protein 1
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: TMIGD1 (transmembrane and immunoglobulin domain containing 1) is a 262 amino acid single-pass membrane protein that contains two Ig-like C2-type (immunoglobulin-like) domains (1, 2) . It is a nonsecreted, glycosylated protein located in the cytoplasm and cell membrane (1) . TMIGD1 might be a possible cell-differentiation marker in the intestine, whose expression is lost during cellular transformation and promote progression of tumorigenesis in the intestinal epithelium (2-4) . TMIGD1 has also been postulated to play a role in cell adhesion (2) .