Product Description
Histone H2AX Antibody | 15-732 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat, Monkey
Homology: N/A
Immunogen: Recombinant protein of human Histone H2AX
Research Area: Other
Tested Application: WB, IHC, ICC
Application: WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
ICC: 1:50 - 1:200
Specificiy: N/A
Positive Control 1: 293T
Positive Control 2: COS-7
Positive Control 3: MCF7
Positive Control 4: K-562
Positive Control 5: T47D
Positive Control 6: PC-3
Molecular Weight: Observed: 17kDa
Validation: N/A
Isoform: N/A
Purification: Affinity purification
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration: N/A
Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.
Alternate Name: H2AFX, H2A.X, H2A/X, H2AX
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.