Product Description
RAB8A Antibody | 19-105 | ProSci
Host: Rabbit
Reactivity: Human
Homology: N/A
Immunogen: Recombinant protein of human RAB8A
Research Area: Cell Cycle, Signal Transduction
Tested Application: WB, IHC, Flow
Application: WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
Flow: 1:20 - 1:50
Specificiy: N/A
Positive Control 1: U-87MG
Positive Control 2: HepG2
Positive Control 3: 293T
Positive Control 4: Mouse liver
Positive Control 5: Mouse brain
Positive Control 6: N/A
Molecular Weight: Observed: 24kDa
Validation: N/A
Isoform: N/A
Purification: Affinity purification
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.02% sodium azide, pH7.3.
Concentration: N/A
Storage Condition: Store at 4˚C. Avoid freeze / thaw cycles.
Alternate Name: RAB8A, MEL, RAB8
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1.