Product Description
RAD17 Antibody | 19-613 | ProSci
Host: Rabbit
Reactivity: Human
Homology: N/A
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 391-670 of human RAD17 (NP_002864.1) .
Research Area: Other
Tested Application: WB, IF, IP
Application: WB: 1:500 - 1:2000
IF: 1:10 - 1:100
IP: 1:50 - 1:200
Specificiy: N/A
Positive Control 1: SW480
Positive Control 2: BT-474
Positive Control 3: K-562
Positive Control 4: 22Rv1
Positive Control 5: Raji
Positive Control 6: N/A
Molecular Weight: Observed: 77kDa
Validation: N/A
Isoform: N/A
Purification: Affinity purification
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration: N/A
Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.
Alternate Name: RAD17, CCYC, HRAD17, R24L, RAD17Sp, Rad24, RAD24, RAD17SP
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC) , and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.