Product Description
StrepII-tag Antibody | 4217 | ProSci
Host: Rabbit
Reactivity: Tag
Homology: N/A
Immunogen: StrepII-tag antibody was raised against the amino acid sequence "gggWSHPQFEK".
Research Area: Other
Tested Application: E, WB
Application: StrepII-tag antibody can be used for the detection of StrepII by Western blot at 0.25 μg/mL.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 100 kDa
Observed: 95 kDa
Validation: N/A
Isoform: N/A
Purification: StrepII-tag Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: StrepII-tag Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: StrepII-tag antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: StrepII-tag Antibody:
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: StrepII-tag Antibody: Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag is a nine amino acid peptide with high specificity and affinity towards streptavidin. The addition of this tag to the C-terminus of recombinant proteins allowed the simple purification of protein by use of affinity columns. It was re-engineered to allow it to also placed at the N-terminus of recombinant proteins.