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Product Description
A novel agent for fast and sensitive detection of DNA synthesis in vivo. 5-EdU is incorporated into proliferating cells followed by detection with a fluorescent azide using click ligation. In contrast to the use of BrdU, the new method does not require sample fixation or DNA denaturation. May be used for studying cell proliferation in the central nervous system and may be combined with BrdU staining in a novel protocol allowing for double labeling of DNA synthesis. EdU incorporation is rapidly becoming the gold standard method for DNA labeling.
A novel agent for fast and sensitive detection of DNA synthesis in vivo. 5-EdU is incorporated into proliferating cells followed by detection with a fluorescent azide using click ligation. In contrast to the use of BrdU, the new method does not require sample fixation or DNA denaturation. May be used for studying cell proliferation in the central nervous system and may be combined with BrdU staining in a novel protocol allowing for double labeling of DNA synthesis. EdU incorporation is rapidly becoming the gold standard method for DNA labeling.
Biovision | 9565 | 5-Ethynyl-2'-deoxyuridine DataSheet
Alternate Name/Synonyms: 2′-Deoxy-5-ethynyluridine; 5-EdU
Appearance: White to off-white solid
Formulation: N/A
CAS Number: 61135-33-9
Structure Available?: Yes
Peptide sequence: N/A
Salt Form: No
Molecular Formula: C₁₁H₁₂N₂O₅
Molecular Weight: 252.22
Cell-Permeable?: Yes
Purity: ≥98% by HPLC
Solubilities: DMSO (~10 mg/ml)
Handling: Protect from air and light
Country of Origin: USA
Tag Line: A DNA synthesis probe
MDL Number: MFCD01675687
PubChem CID: 472172
SMILES: C#CC1=CN(C(=O)NC1=O)C2CC(C(O2)CO)O
InChi: InChI=1S/C11H12N2O5/c1-2-6-4-13(11(17)12-10(6)16)9-3-7(15)8(5-14)18-9/h1,4,7-9,14-15H,3,5H2,(H,12,16,17)/t7-,8+,9+/m0/s1
InChi Key: CDEURGJCGCHYFH-DJLDLDEBSA-N
