Product Description
DFHBI-1T is a mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in living cells. Fluorescence is activated by binding to Spinach2 or Broccoli aptamers. Displays peak excitation maxima of 482 nm and peak fluorescence emission of 505 nm when bound to Spinach2, enabling imaging with GFP filter cubes. DFHBI-1T has been found to have lower background fluorescence than DFHBI (Cat. No. B2797) and exhibits an overall increase in brightness in living cells. DFHBI-1T is cell-permeable with negligible toxicity in living cells and can be used to label any genetically encoded Spinach™, Spinch2™, and Broccoli™ RNA tags.
DFHBI-1T is a mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in living cells. Fluorescence is activated by binding to Spinach2 or Broccoli aptamers. Displays peak excitation maxima of 482 nm and peak fluorescence emission of 505 nm when bound to Spinach2, enabling imaging with GFP filter cubes. DFHBI-1T has been found to have lower background fluorescence than DFHBI (Cat. No. B2797) and exhibits an overall increase in brightness in living cells. DFHBI-1T is cell-permeable with negligible toxicity in living cells and can be used to label any genetically encoded Spinach™, Spinch2™, and Broccoli™ RNA tags.
Biovision | B2799 | DFHBI-1T DataSheet
Alternate Name/Synonyms: DFHBI1T,(Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-1-(2,2,2-trifluoroethyl)-1H-imidazol-5(4 H)-one
Appearance: Yellow solid
Formulation:
CAS Number: 1539318-36-9
Structure Available?: Yes
Peptide sequence: N/A
Salt Form: No
Molecular Formula: C₁₃H₉F₅N₂O₂
Molecular Weight: 320.21
Cell-Permeable?: TRUE
Purity: ≥98% by HPLC
Solubilities: >15 mg/ml Ethanol, >30 mg/ml DMSO
Handling: Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.
Country of Origin: USA
Tag Line: A GFP fluorophore mimic for imaging RNA in living cells
MDL Number: MFCD31628972
PubChem CID: 101889712
SMILES: CC1=NC(=CC2=CC(=C(C(=C2)F)O)F)C(=O)N1CC(F)(F)F
InChi: InChI=1S/C13H9F5N2O2/c1-6-19-10(12(22)20(6)5-13(16,17)18)4-7-2-8(14)11(21)9(15)3-7/h2-4,21H,5H2,1H3/b10-4-
InChi Key: AWYCLBWNRONMQC-WMZJFQQLSA-N