Product Description
DFHBI is a cell-permeable non-fluorescent small molecule derived from the GFP chromophore. Upon binding to the “Spinach™” aptamer, DFHBI is converted to a highly fluorescent state that can be detected at the emission wavelength of 501 nm. DFHBI displays negligible toxicity in living cells. DFHBI can be used to label any genetically encoded Spinach™ family of RNA tags that include Spinach™, Spinach2™, and Broccoli™. Live-cell imaging of tagged RNA can be performed using a standard fluorescence microscopy. Further, DFHBI can also selectively detect tagged RNA in total RNA gel electrophoresis.
DFHBI is a cell-permeable non-fluorescent small molecule derived from the GFP chromophore. Upon binding to the “Spinach™” aptamer, DFHBI is converted to a highly fluorescent state that can be detected at the emission wavelength of 501 nm. DFHBI displays negligible toxicity in living cells. DFHBI can be used to label any genetically encoded Spinach™ family of RNA tags that include Spinach™, Spinach2™, and Broccoli™. Live-cell imaging of tagged RNA can be performed using a standard fluorescence microscopy. Further, DFHBI can also selectively detect tagged RNA in total RNA gel electrophoresis.
Biovision | B2797 | DFHBI DataSheet
Alternate Name/Synonyms: (5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2,3-dimethyl- 4H-Imidazol-4-one,3’5’-difluoro-4-hydroxybenzylidene
Appearance: Yellow solid
Formulation:
CAS Number: 1241390-29-3
Structure Available?: Yes
Peptide sequence: N/A
Salt Form: No
Molecular Formula: C₁₂H₁₀F₂N₂O₂
Molecular Weight: 252.22
Cell-Permeable?: TRUE
Purity: ≥95% by HPLC
Solubilities: ~20 mg/ml DMSO
Handling: Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.
Country of Origin: USA
Tag Line: A cell-permeable, non-toxic fluorogen
MDL Number:
PubChem CID: 70808995
SMILES: CC1=NC(=CC2=CC(=C(C(=C2)F)O)F)C(=O)N1C
InChi: InChI=1S/C12H10F2N2O2/c1-6-15-10(12(18)16(6)2)5-7-3-8(13)11(17)9(14)4-7/h3-5,17H,1-2H3/b10-5-
InChi Key: ZDDIJYXDUBFLID-YHYXMXQVSA-N