Product Description
Fluo-4 is a widely used green fluorescent, intracellular calcium (Ca²⁺) indicator. Fluo-4 AM is the membrane permeable form of the calcium indicator. Masking the negative charge using non-polar, ester-linked moieties (AM ester) allows the molecule to enter cells through passive diffusion. Once inside the cell, ubiquitous intracellular esterase enzymes promote rapid hydrolysis of the AM ester leaving the active, highly polar form of the indicator trapped inside the cell. It binds to calcium with a Kd value of 355 nM and displays excitation/emission maxima of 490/515 nm, respectively. It is used to measure intracellular Ca²⁺ flux in high throughput screening and fluorescence microscopy applications.
Fluo-4 is a widely used green fluorescent, intracellular calcium (Ca²⁺) indicator. Fluo-4 AM is the membrane permeable form of the calcium indicator. Masking the negative charge using non-polar, ester-linked moieties (AM ester) allows the molecule to enter cells through passive diffusion. Once inside the cell, ubiquitous intracellular esterase enzymes promote rapid hydrolysis of the AM ester leaving the active, highly polar form of the indicator trapped inside the cell. It binds to calcium with a Kd value of 355 nM and displays excitation/emission maxima of 490/515 nm, respectively. It is used to measure intracellular Ca²⁺ flux in high throughput screening and fluorescence microscopy applications.
Biovision | B3146 | Fluo-4 AM DataSheet
Alternate Name/Synonyms: Calcium (Ca2⁺) indicator; Fluo-4 membrane permeable; Fluo-4 acetoxymethyl ester; Fluorescent Calcium indicator
Appearance: Red orange powder
Formulation:
CAS Number: 273221-67-3
Structure Available?: TRUE
Peptide sequence:
Salt Form: FALSE
Molecular Formula: C₅₁H₅₀F₂N₂O₂₃
Molecular Weight: 1097
Cell-Permeable?: TRUE
Purity: ≥ 95%
Solubilities: ~1 mg/ml in DMSO
Handling: Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.
Country of Origin: USA
Tag Line: A membrane permeable fluorescent calcium indicator
MDL Number:
PubChem CID: 4060965
SMILES: CC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC2=C(C=CC(=C2)C3=C4C=C(C(=O)C=C4OC5=CC(=C(C=C53)F)OCOC(=O)C)F)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C
InChi: InChI=1S/C51H50F2N2O23/c1-28-7-9-39(54(19-47(62)74-24-69-30(3)57)20-48(63)75-25-70-31(4)58)45(13-28)66-11-12-67-46-14-34(8-10-40(46)55(21-49(64)76-26-71-32(5)59)22-50(65)77-27-72-33(6)60)51-35-15-37(52)41(61)17-42(35)78-43-18-44(38(53)16-36(43)51)73-23-68-29(2)56/h7-10,13-18H,11-12,19-27H2,1-6H3
InChi Key: QOMNQGZXFYNBNG-UHFFFAOYSA-N