Product Description
Cathepsin D is a lysosomal aspartyl protease composed of a protein dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is an estrogen-regulated protein associated with tissue breakdown. Levels of cathepsin D have been positively correlated with recurring breast cancers of both node negative and node positive types. Additionally cathepsin D has been associated with amyloid formation in Alzheimers plaques. Cathepsin D is produced initially as a pre-pro-enzyme which gets transported to lysosomes via endosomes in most cell types. In endosomes, it gets proteolyzed by unidentified proteases by removal of the pro-peptide to generate active single-chain Cathepsin D; while in lysosomes, further processing by cysteine cathepsins B and L generates mature, active double-chain Cathepsin D. BioVisions Active Human Cathepsin D is a proteolytically active, non-glycosylated enzyme expressed, purified and activated by proprietary methods.
Biovision | 9229 | Active Cathepsin D Human Recombinant DataSheet
Biomolecule/Target: Cathepsin D
Synonyms: Procathepsin D, CTSD
Alternates names: Procathepsin D, CTSD
Taglines: An active Lysosomal aspartyl protease
NCBI Gene ID #: 8744
NCBI Gene Symbol: TNFL9
Gene Source: Human
Accession #: P41273
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: 80%
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): Measured by Cathepsin D Activity Fluorometric Assay Kit and Pepstatin A inhibition using Cathepsin D inhibitor screening kit
Biological activity: Determined by the dose-dependent stimulation of IL-8 production by human PBMC. The ED for the effect is less than 10 ng/ml. Please note: results may vary with PBMC donors.
Results: BV's Cathepsin D has specific activity >100 pmol/min/mg and IC50 = 0.32 pM
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 19.5 kDa
Concentration: N/A
Appearance: Freeze-Dried
Physical form description: Freeze-dried from proprietary buffer
Reconstitution Instructions: We recommend a quick spin followed by reconstitution in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or 20°C for future use.
Amino acid sequence: N/A