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Product Description
D-2-hydroxyglutarate (D2HG) level is significantly increased in metabolic diseases and various cancers such as acute myeloid leukemia. Studies suggest that the detection of D2HG serves as a biomarker assay related to IDH (isocitrate dehydrogenase) mutations. D2HGDH is a special NAD-dependent enzyme which reacts with D2HG specifically and converts D2HG to -ketoglutarate. D2HGDH is a key enzyme to distinguish between two metabolites, D2HG and L-2-hydroxyglutarate (L2HG), during biomarker assays.
Biovision | P1001 | Active D-2-Hydroxyglutaratate Dehydrogenase D2HGDH Recombinant DataSheet
Biomolecule/Target: D2HGDH
Synonyms: D-2-hydroxyglutarate dehydrogenase, D2HGDH, D2HGD
Alternates names: D-2-hydroxyglutarate dehydrogenase, D2HGDH, D2HGD
Taglines: D2HGDH is an active protein that catalyzes the oxidation of D-2-hydroxyglutarate to alpha-ketoglutarate.
NCBI Gene ID #: 3604
NCBI Gene Symbol: TNR9
Gene Source: Acidaminococcus fermentans
Accession #: Q07011
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): The specific activity of D2HGDH is 90,000 mU/mg. (Specific activity is tested by the D-2-Hydroxyglutarate Colorimetric Assay Kit (Cat. No. 213-100) (Unit Definition: One unit is the amount of enzyme that reduces 1.0 µmole of NAD+ to NADH per min. at pH 8 at 37°C.)
Biological activity: Determined by its inhibitory effect of the 4-1BBL mediated stimulation of IL-8 production by human PBMC. About 90% of inhibition was seen using a concentration of 1 µg for both 4-1BBL and 4-1BBR.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 17.8 kDa
Concentration: N/A
Appearance: Dry powder
Physical form description: Lyophilized powder
Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or 20°C for future use.
Amino acid sequence: N/A
