Product Description
Caspases are known to play an important role in apoptosis. Caspases exist in cells as inactive pro-enzymes and mature by proteolysis to yield large and small subunits. Active caspases are heterotetramers consisting of two large and two small subunits.
Biovision | K230 | Active Human Caspases Set I DataSheet
Biomolecule/Target: N/A
Synonyms: Active Human Caspases Set I
Alternates names: N/A
Taglines: Set of recombinant proteases involved in apoptosis
NCBI Gene ID #: CASP3- 836 CASP6- 839 CASP7- 840 CASP8- 841
NCBI Gene Symbol: CASP3 CASP6 CASP7 CASP8
Gene Source: Human
Accession #: CASP3- P42574 CASP6- P55212 CASP7- P55210 CASP8- Q14790
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: N/A
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: N/A
Results: N/A
Binding Capacity: N/A
Unit Definition: One unit of the recombinant human caspase-3, -6, -7, and -8 is the enzyme activity that cleaves 1 nmol of the caspase substrate DEVD-pNA, VEID-pNA, DEVD-pNA, and IETD-pNA, respectively, per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
Molecular Weight: N/A
Concentration: N/A
Appearance: Lyophilized
Physical form description: Lyophilized powder
Reconstitution Instructions: Reconstitute to 0.1-1 unit per µl in PBS + 15% glycerol
Amino acid sequence: N/A