Product Description
Caspases are known to play an important role in apoptosis. Caspases exist in cells as inactive pro-enzymes. Most of the pro-caspases are matured by proteolysis to yield large and small subunits. Active caspases are heterotetramers, each of which consists of two large and two small subunits.
Biovision | K231 | Active Human Caspases Set II DataSheet
Biomolecule/Target: N/A
Synonyms: Active Human Caspases Set II
Alternates names: N/A
Taglines: Set of recombinant proteases involved in apoptosis
NCBI Gene ID #: CASP1- 834 CASP2- 835 CASP9- 842 CASP10- 843
NCBI Gene Symbol: CASP1 CASP2 CASP9 CASP10
Gene Source: Human
Accession #: CASP1-NP_150634.1 CASP2-P42575 CASP9-P55211 CASP10-Q92851
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: N/A
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: N/A
Results: N/A
Binding Capacity: N/A
Unit Definition: One unit of the recombinant human caspase-1, -2, -9, and -10 is the enzyme activity that cleaves 1 nmol of the caspase substrate YVAD-pNA, VDVAD-pNA, LEHD-pNA, and IETD-pNA, respectively, per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
Molecular Weight: N/A
Concentration: N/A
Appearance: Lyophilized
Physical form description: Lyophilized powder
Reconstitution Instructions: Reconstituted to 0.1-1 unit per µl in PBS.
Amino acid sequence: N/A