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Product Description
Human Sirtuins function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. Sirtuin 6 (SIRT6) is a NAD-dependent protein deacetylase with activity towards Lys-9 and Lys-56 of histone H3. It modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. It is required for genomic stability and for normal IGF1 serum levels and normal glucose homeostasis. It also regulates cellular senescence and apoptosis. Unlike most sirtuins, SIRT6 preferentially removes long-chain fatty-acyl, rather than acetyl, protein modifications. This activity regulates secretion of the inflammation-associated protein TNF-.
Biovision | 7697 | Active SIRT6 GST-tagged human recombinant DataSheet
Biomolecule/Target: SIRT6
Synonyms: SIRT6, NAD-dependent Deacetylase Sirtuin-6; Mono-ADP-ribosyltransferase Sirtuin 6; SIR2-like Protein 6; SIR2L6
Alternates names: SIRT6, NAD-dependent Deacetylase Sirtuin-6; Mono-ADP-ribosyltransferase Sirtuin 6; SIR2-like Protein 6; SIR2L6
Taglines: GST tagged Human SIRT6 from E.Coli, AOF, 85% pure, biologically active.
NCBI Gene ID #: 50498
NCBI Gene Symbol: EBI3
Gene Source: Human
Accession #: Q3U1K3
Recombinant: Yes
Source: E. coli.
Purity by SDS-PAGEs: 90%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): BioVisions Active GST-tagged SIRT6 has been tested for its activity using Sirtuin Activity Assay Kit (K324-100). It has a specific activity of >3 mU/mg (One unit of Sirtuin activity is the amount of enzyme that hydrolyzes the substrate to yield 1 µmol of AFC/min at 37°C).
Biological activity: Assay data for mouse recombinant EBI-3 is based upon qualitative binding to anti-EBI-3 antibody.
Results: >3 mU/mg
Binding Capacity: N/A
Unit Definition: One unit of Sirtuin activity is the amount of enzyme that hydrolyzes the substrate to yield 1 µmol of AFC/min at 37°C
Molecular Weight: 22.9 kDa
Concentration: 1 mg/ml
Appearance: Liquid
Physical form description: Human SIRT6 protein is a solution of 1 mg/ml in 50 mM Tris, 100 mM NaCl, pH 8.0 containing 20% glycerol.
Reconstitution Instructions: When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile HO at a concentration of 0.1 0.5 mg/ml, which can be further diluted into other aqueous solutions.
Amino acid sequence: N/A
