HIV-1 protease is a retroviral aspartyl protease (retropepsin) that is essential for the life-cycle of HIV. HIV protease cleaves newly synthesized viral polyproteins at the appropriate places into functional protein products as mature protein components of an infectious HIV virion. The mutation of HIV protease's active site or inhibition of its activity disrupts HIVs ability to replicate and infect additional cells.
Biomolecule/Target: HIV1 Protease
Synonyms: HIV-1 retropepsin, HIV-1 Protease (PR), cd05482
Alternates names: HIV-1 retropepsin, HIV-1 Protease (PR), cd05482
Taglines: 90% Pure Active Recombinant HIV1 Protease (GST tagged) for inhibitor screening assays, activity studies, selectivity profiling, western blotting, ELISA, and numerous such applications.
NCBI Gene ID #: 3604
NCBI Gene Symbol: TNR9
Gene Source: HIV1
Accession #: Q07011
Source: E. coli
Purity by SDS-PAGEs: 98%
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: Determined by its inhibitory effect of the 4-1BBL mediated stimulation of IL-8 production by human PBMC. About 90% of inhibition was seen using a concentration of 1 µg for both 4-1BBL and 4-1BBR.
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 17.8 kDa
Concentration: 3 mg/ml
Physical form description: 3 mg/ml in 50 mM Sodium acetate, 100 mM NaCl, 5 mM DTT, 5 mM EDTA, pH 5.0 containing 10% glycerol
Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or 20°C for future use.
Amino acid sequence: N/A