Product Description
ANG-2 binds to the endothelial cell specific receptor Tie2, but, in contrast to ANG-1 does not induce tyrosine phosphorylation. Consequently, ANG-2 modulates ANG-1 activation of Tie2 and, depending on the physiological and biochemical environment, can act either as a n agonist or antagonist of Tie2 induced angiogenesis. The signaling interactions of ANG-1, ANG-2 and Tie2, along with less characterized ANG-3 and ANG-4, are required for embryonic and adult angiogenesis. Physiologically, ANG-1 and ANG-2 are associated with sprouting, tube formation, and structural integrity of newly formed blood vessels. Mature human ANG-2 is a secreted protein containing 480 amino acid residues. ANG-2 is composed of an alpha helix rich coiled coil N-terminal domain and fibrinogen like C-terminal domain. ANG-2 exists predominantly in the form of a disulfide-linked dimer. Recombinant human ANG-2 is a C-terminal histidine tagged glycoprotein which migrates with an apparent molecular mass of 60.0 70.0 kDa by SDS-PAGE under reducing conditions. Sequencing analysis shows an N-terminal sequence starting with residue 68 (D) of the ANG-2 precursor protein.
Biovision | 7116 | ANG-2 Human Recombinant DataSheet
Biomolecule/Target: ANG-2
Synonyms: ANGPT-2
Alternates names: ANGPT-2
Taglines: Promotes angiogenesis during the development, remodeling, and repair of the vascular system
NCBI Gene ID #:
NCBI Gene Symbol: TNFSF13B
Gene Source: Human
Accession #: D4AAC1
Recombinant: Yes
Source: CHO cells
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).
Activity (Specifications/test method): Determined by its ability to stimulate tubulogenesis in HUVEC cells using a concentration of 0.2 g/ml.
Biological activity: N/A
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 20.7 kDa
Concentration: N/A
Appearance: Lyophilized powder
Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 8.0.
Reconstitution Instructions: Reconstitute in sterile HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers or stored at 4°C for 1 week or 20°C for future use.
Amino acid sequence: N/A