Product Description
Calcium/calmodulin-dependent protein kinase ID (CAMK1D), or a novel Ca2+/calmodulin-dependent kinase I-like kinase (CKLiK), showed kinase activity and that the activity was enhanced by Ca(2+) and calmodulin. Using a novel antibody generated against the C-terminus of CKLiK, CKLiK was detected in CD34+-derived neutrophils and eosinophils, as well as in mature peripheral blood granulocytes. Activation of human granulocytes by N-formyl-methionyl-leucyl-phenylalanine (fMLP) and platelet-activating factor (PAF), but not the phorbol ester PMA (phorbol 12-myristate-13-acetate), resulted in induction of CKLiK activity, in parallel with a rise of intracellular [Ca2+]. Furthermore, fMLP-induced neutrophil migration on albumin-coated surfaces was perturbed, as well as 2-integrin-mediated adhesion. These findings suggest a critical role for CKLiK in modulating chemoattractant-induced functional responses in human granulocytes (1). Also, CAMK1D exhibits Ca(2+)/CaM-dependent activity that is enhanced (approximately 30-fold) in vitro by phosphorylation of its Thr180 by CaM-K kinase (CaM-KK), consistent with detection of CAMK1D-activating activity in HeLa cells. Transiently expressed CAMK1D exhibited enhanced protein kinase activity in HeLa cells without ionomycin stimulation. This sustained activation of CAMK1D was completely abolished by Thr180Ala mutation and inhibited by CaM-KK inhibitor, STO-609, indicating a functional CaM-KK/ CAMK1D cascade in HeLa cells.
Biovision | 7713 | CAMK1d Active DataSheet
Biomolecule/Target: N/A
Synonyms: CAMK, Calcium/calmodulin-dependent protein kinase type I delta chain
Alternates names: CAMK, Calcium/calmodulin-dependent protein kinase type I delta chain
Taglines: Involved in many signaling cascades
NCBI Gene ID #: 12370
NCBI Gene Symbol: CASP8
Gene Source: N/A
Accession #: Q3U607
Recombinant: Yes
Source: Baculovirus (Sf9 insect cells)
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): 138 nmol phosphate incorporated into Autocamtide 2 per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 Ci/assay).
Biological activity: N/A
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 60.0 kDa
Concentration: 5µg/50 µl
Appearance: Liquid
Physical form description: Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
Reconstitution Instructions: N/A
Amino acid sequence: N/A