Product Description
Caspase-10/a (also known as Mch4) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-10 also exists in cells as an inactive proenzyme. During apoptosis procaspase-10 is processed at aspartate residues to form active caspase-10 which consists of two large (18 kDa) and two small (11 kDa) subunits in a heterotetramer form. The recombinant active human caspase-10 was expressed in E. coli. The active caspase-10 is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-IETD-pNA (Cat. #1063-200) or Ac-IETD-AFC (1062-200).
Biovision | 1090A | Caspase-10/a human recombinant DataSheet
Biomolecule/Target: Caspase-10/a
Synonyms: Caspase-10/a
Alternates names: IL10, D ZCYTO10, IL-20, MGC96907, Four helix cytokine, Zcyto10,
Taglines: A cysteine protease involved in apoptosis
NCBI Gene ID #: 58181
NCBI Gene Symbol: IL20
Gene Source: Human
Accession #: Q9JKV9
Recombinant: Yes
Source: E. coli.
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): > 8000 units/mg
Biological activity: Mouse IL-20 is fully biologically active when compared to standard. The biological activity was determined by measuring the dose-dependent activation of STAT3 in human epidermal keratinocytes (HEK2a cells). Significant STAT3 activation is observed with >100 ng/ml of recombinant mouse IL-20
Results: N/A
Binding Capacity: N/A
Unit Definition: One unit of the recombinant caspase-10/a is the enzyme activity that cleaves 1 nmol of the caspase substrate IETD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
Molecular Weight: 17.6 kDa
Concentration: N/A
Appearance: Lyophilized powder
Physical form description: Lyophilized powder
Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-0.5 mg/ml (Note: Gently pipetting to mix). The solution can be diluted into other aqueous buffers and stored at 4°C for 1 week or 20°C for future use.
Amino acid sequence: N/A