Product Description
Cathepsin L is a lysosomal protease that can be secreted from the cell and is implicated to play a role in protein degradation, arthritis, apoptosis, and cancer.
Biovision | 1135 | Cathepsin L human recombinant DataSheet
Biomolecule/Target: Cathepsin L
Synonyms: Cathepsin
Alternates names: MIC-1, Placental TGF, Prostate differentiation factor
Taglines: Lysosomal cysteine protease involved in protein degradation, arthritis, apoptosis, and cancer
NCBI Gene ID #: 9518
NCBI Gene Symbol: GDF-15
Gene Source: Human
Accession #: Q99988
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): > 10000 mU/mg (1 U = digestion of 1 µmole of Z-Phe-Arg-AFC substrate (K142-100) per minute at 37°C in 50 mM Sodium Acetate, 100 mM NaCl, pH 5.5).
Biological activity: The activity is determined by the inhibition of DU-145 cells and is typically 1-2 µg/ml.
Results: > 10000 mU/mg
Binding Capacity: N/A
Unit Definition: 1 U = digestion of 1 µmole of Z-Phe-Arg-AFC substrate (K142-100) per minute at 37°C in 50 mM Sodium Acetate, 100 mM NaCl, pH 5.5
Molecular Weight: 26.8 kDa
Concentration: 0.5 mg/ml
Appearance: Liquid
Physical form description: 0.5 mg/ml in 25 mM Sodium Acetate, 50 mM NaCl, pH 5.5 containing 50% glycerol
Reconstitution Instructions: Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile 5 mM acetic acid at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Amino acid sequence: MARNGDDCPL GPGRCCRLHT VRASLEDLGW ADWVLSPREV QVTMCIGACP SQFRAANMHA QIKTSLHRLK PDTVPAPCCV PASYNPMVLI QKTDTGVSLQ TYDDLLAKDC HCI