Product Description
CD3E is the CD3-epsilonpolypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. Recombinant human CD3E protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Biovision | 7342 | CD3E human recombinant DataSheet
Biomolecule/Target: CD3E
Synonyms: T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE
Alternates names: T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE
Taglines: Plays a role in coupling antigen recognition to several intracellular signal-transduction pathways
NCBI Gene ID #: 6469
NCBI Gene Symbol: SHH
Gene Source: Human
Accession #: Q15465
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 97%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: N/A
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 14.1 kDa (127 aa, 23-126 aa + His Tag), confirmed by MALDI-TOF.
Concentration: 1 mg/ml
Appearance: Liquid
Physical form description: 1 mg/ml in 20 mM Tris-HCl buffer (pH 8.0) containing 0.2 M NaCl, and 10% glycerol.
Reconstitution Instructions: N/A
Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MGSDGNEEMG GITQTPYKVS ISGTTVILTC PQYPGSEILW QHNDKNIGGD EDDKNIGSDE DHLSLKEFSE LEQSGYYVCY PRGSKPEDAN FYLYLRARVC ENCMEMD