Product Description
The recombinant CFP (Cyan Fluorescent Protein) is expressed and purified from transformed E. coli using a method that ensures high purity and maximal CFP fluorescence. Endotoxin has been removed, so the protein is suitable for in vivo injection or cell culture applications. The protein is a 31.3 kDa monomer with 284 amino acids and an N-terminal His-tag, Ex./Em. = 458/480 nm, extinction coefficient 26000M-1CM-1.
Biovision | 4996 | Cyan Fluorescent Protein DataSheet
Biomolecule/Target: N/A
Synonyms: Recombinant CFP Protein, Cyan Fluoroscent Protein
Alternates names: bone morphogenetic protein 10, BMP10, BMP 10
Taglines: Fluorescent protein ideal for subcellular labeling/visualization
NCBI Gene ID #: 27302
NCBI Gene Symbol: BMP10
Gene Source: N/A
Accession #: O95393
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: 97%
Assay #2: HPLC
Endotoxin Level: <0.1 ng/g
Activity (Specifications/test method): N/A
Biological activity: The protein is biologically active as determined by its ability to induce alkaline phosphatase production by C2C12 cells. The ED is in the range of 0.3-3 µg/ml.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 32 kDa
Concentration: N/A
Appearance: Lyophilized protein
Physical form description: Freeze Dried
Reconstitution Instructions: Reconstitute to a concentration of 0.1-1.0 mg/ml in HO containing BSA (50 mg BSA per 1 mg of protein). This solution can then be diluted into other aqueous buffers
Amino acid sequence: N/A