Product Description
CDA (Cytidine deaminase) is an enzyme that scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis. This protein is one of several deaminases responsible for maintaining the cellular pyrimidine pool. The protein also catalyzes the deamination of chemotherapeutic cytosine nucleoside analogs such as Ara-C and 5-azacytidine, which results in the loss of their cytotoxic and antitumor function. It can form homotetramers and is mainly expressed in granulocytes. Recombinant human CDA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Biovision | 7363 | Cytidine deaminase human recombinant DataSheet
Biomolecule/Target: Cytidine Deaminase
Synonyms: CDA, CDD, Cytidine aminohydrolase, Cytosine nucleoside deaminase
Alternates names: CDA, CDD, Cytidine aminohydrolase, Cytosine nucleoside deaminase
Taglines: An enzyme that scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis
NCBI Gene ID #: UniGeneHs.419815
NCBI Gene Symbol: EGF
Gene Source: Human
Accession #: Q6QBS2
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: N/A
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 18.3 kDa (166 aa, 1-146 aa + His Tag).
Concentration: 0.5 mg/ml
Appearance: Liquid
Physical form description: 0.5 mg/ml in 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 1 mM DTT, 2 mM EDTA and 40% glycerol.
Reconstitution Instructions: N/A
Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MAQKRPACTL KPECVQQLLV CSQEAKQSAY CPYSHFPVGA ALLTQEGRIF KGCNIENACY PLGICAERTA IQKAVSEGYK DFRAIAIASD MQDDFISPCG ACRQVMREFG TNWPVYMTKP DGTYIVMTVQ ELLPSSFGPE DLQKTQ