Euro
USD
British Pound
NULL
Product Description
Carbonic anhydrase (CA) is an enzyme that catalyses rapid conversion of carbon dioxide to bicarbonate and protons (CO2 + H2O HCO3 + H+). Most carbonic anhydrases contain a zinc ion in their active site and the primary function of this enzyme is known to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide of tissues. Carbonic anhydrases have been found in all kingdoms of life. Recombinant carbonic anhydrase fused to His-tag, was expressed in E.coli and purified by conventional chromatography techniques.
Biovision | P1049 | E. coli Recombinant Carbonic anhydrase DataSheet
Biomolecule/Target: can
Synonyms: Carbonate dehydratase, CAN, yadF
Alternates names: Leukocyte interferon, B cell interferon, Type I interferon, IFNA2, IFN- 2a
Taglines: Catalyzes the reversible hydration of carbon dioxide in various biological processes.
NCBI Gene ID #: 3440
NCBI Gene Symbol: IFNA2
Gene Source: E. coli
Accession #: P01563
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): Measured by its ability to hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.
Biological activity: The specific activity as determined in a viral resistance assay using VSV-WISH cells was found to be greater than 1 x 10 IU/mg
Results: >1,000 pmol/min/ug
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 19.4 kDa
Concentration: N/A
Appearance: Liquid
Physical form description: In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol
Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1 mg/ml. This solution can then be diluted into other aqueous buffers
Amino acid sequence: N/A
