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Product Description
Exonuclease I hydrolyzes single-stranded DNA in the 3'5' direction, releasing 5'-mononucleotides and leaving the terminal 5'-dinucleotide intact. Hydrolysis is processive and cannot proceed if the 3' terminus is phosphorylated.
Biovision | 9217 | Exonuclease I DataSheet
Biomolecule/Target: N/A
Synonyms: Exo I
Alternates names: Exo I
Taglines: Hydrolyzes single-stranded DNA in the 3'5' direction, releasing 5'-mononucleotides and leaving the terminal 5'-dinucleotide intact.
NCBI Gene ID #: 2247
NCBI Gene Symbol: FGF2
Gene Source: N/A
Accession #: P09038
Recombinant: Yes
Source: E. coli strain containing an overproducing clone of E. coli Exonuclease I.
Purity by SDS-PAGEs: 97%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): 10 unit/l
Biological activity: N/A
Results: N/A
Binding Capacity: N/A
Unit Definition: One unit is the amount of enzyme which catalyzes the release of 10 nmol of acid-soluble nucleotide from denatured DNA in 30 min at 37°C under standard conditions.
Molecular Weight: 55.0 kDa
Concentration: N/A
Appearance: Liquid
Physical form description: Exonuclease I in 20 mM Tris-HCI (pH 7.5), 5 mM 2-mercaptoethanol, 0.5 mM EDTA, 50% glycerol.
Reconstitution Instructions: N/A
Amino acid sequence: N/A
