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Product Description
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a catalytic enzyme commonly known to be involved in glycolysis. The enzyme exists as a tetramer composed of 36-kDa subunits and has various intracellular functions. GAPDH catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Besides functioning as a glycolytic enzyme in cytoplasm, evidence suggests that mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair. Recombinant GAPDH protein was expressed in E.coli and purified by using conventional chromatography techniques.
Biovision | P1100 | GAPDH Active human recombinant DataSheet
Biomolecule/Target: GAPDH
Synonyms: Glyceraldehyde-3-phosphate dehydrogenase, G3PD, GAPD
Alternates names: Mammotropin, Luterotropic hormone, Lutetropin, Prlr, PRL, luteotropic hormone, LTH, PRLR, PRL-R, PRL R
Taglines: Involved in glycolysis
NCBI Gene ID #: 24683
NCBI Gene Symbol: PRL
Gene Source: Human
Accession #: P01237
Recombinant: Yes
Source: E. Coli
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): Measured by its ability to convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C
Biological activity: The ED determined by the dose-dependent stimulation of the proliferation of rat lymphoma, Nb2-11 cells was found to be in the range of 0.1-1.0 ng/ml.
Results: > 90 units/mg
Binding Capacity: N/A
Unit Definition: One unit is defined as the amount of enzyme that convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C.
Molecular Weight: 22.5 kDa
Concentration: 1 mg/ml
Appearance: Liquid
Physical form description: In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 1 mM EDTA, 20% glycerol
Reconstitution Instructions: Reconstitute in HO to a concentration of 1.0 mg/ml. This solution can then be diluted into other aqueous buffers
Amino acid sequence: N/A
