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Product Description
Glucose is phosphorylated to glucose-6-phosphate by glucokinases. This gene is alternatively spliced to generate three different forms of the enzyme; one found in the pancreas and two found in the liver. The main function of this gene is to regulate carbohydrate metabolism. Recombinant human pancreatic Glucokinase (Accession# NP_000153)) has a C-terminal FLAG tag and has 470 amino acid residues. It can be useful for studies including enzyme kinetics, activator screening and kinase selectivity.
Biovision | 7777 | Glucokinase human pancreatic recombinant DataSheet
Biomolecule/Target: N/A
Synonyms: N/A
Alternates names: Glucokinase, Hexokinase type IV, HK IV, Hexokinase-4, HK4, Hexokinase-D
Taglines: Plays an important role in the regulation of carbohydrate metabolism
NCBI Gene ID #: 27006
NCBI Gene Symbol: FGF-22
Gene Source: Human pancreas
Accession #: Q9HCT0
Recombinant: Yes
Source: E. coli.
Purity by SDS-PAGEs: 97%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): 303 pmol/min/µg. One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 30oC. Assay conditions: 25 mM HEPES, pH 7.5, 2 mM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2.0 mM ATP, 25 mM glucose, 100 µg/ml BSA, 20 units/ml glucose 6-phosphate dehydrogenase, and 10 nM human pancreatic glucokinase at 30°C for 30 min.
Biological activity: N/A
Results: N/A
Binding Capacity: N/A
Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 30oC. Assay conditions: 25 mM HEPES, pH 7.5, 2 mM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2.0 mM ATP, 25 mM glucose, 100 µg/ml BSA, 20 units/ml glucose 6-phosphate dehydrogenase, and 10 nM human pancreatic glucokinase at 30oC for 30 min.
Molecular Weight: 53.1 kDa
Concentration: 39.3 mg/ml
Appearance: Liquid
Physical form description: 50 mM potassium phosphate pH 7.4, 50 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, and 2.5% glycerol.
Reconstitution Instructions: N/A
Amino acid sequence: N/A
