Product Description
Granzyme B (GZMB) is a serine protease stored in granules of activated cytotoxic T cells and NK cells. Upon target cell contact, granzyme B is directionally exocytosed and assisted by perforin, enters the target cell. With its unique substrate specificity (granzyme B cleaves after Asp), granzyme B processes and activates various pro-caspases thereby inducing apoptosis in the target cell.
Biovision | 4728 | Granzyme B human recombinant Insect DataSheet
Biomolecule/Target: N/A
Synonyms: GranzymeB, Granzyme-2, T-cell serine protease 1-3E, Cytotoxic T-lymphocyte proteinase 2, Lymphocyte protease, SECT, Cathepsin G-like 1, CTSGL1, CTLA-1, Fragmentin-2, Human lymphocyte protein, HLP, C11
Alternates names: GranzymeB, Granzyme-2, T-cell serine protease 1-3E, Cytotoxic T-lymphocyte proteinase 2, Lymphocyte protease, SECT, Cathepsin G-like 1, CTSGL1, CTLA-1, Fragmentin-2, Human lymphocyte protein, HLP, C11
Taglines: A serine protease involved in CTL mediated apoptosis
NCBI Gene ID #: 56636
NCBI Gene Symbol: FGF-21
Gene Source: Human
Accession #: Q9JJN1
Recombinant: Yes
Source: Insect cells
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: Activity of GZMB was tested using the synthetic peptide, IETD-AFC, as a substrate. GZMB releases AFC and then the GZMB activity can be quantified by fluorescent detection of free AFC at Ex/Em=380/500 nm.
Results: 40 units/g
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 32 kDa
Concentration: N/A
Appearance: Lyophilized protein
Physical form description: Lyophilized powder from 0.22 m filtered solution in PBS containing 8% trehalose
Reconstitution Instructions: Use 50 µl sterile D.I. water to dissolve 5 µg protein to a final conc of 0.1 µg protein/µl.
Amino acid sequence: N/A