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Product Description
Glycogen synthase kinase-3 (GSK-3) is a serine/theronine kinase that was first characterized by its role in intermediary metabolism. In mammals, serine kinase is encoded by two related genes called GSK-3 (51 kDa) and GSK-3 (46 kDa). GSK-3 has been implicated in the regulation of the c-Jun/AP-1 transcription factor and other intracellular signaling pathways.
Biovision | 7004 | GSK-3 human recombinant DataSheet
Biomolecule/Target: N/A
Synonyms: GSK, Glycogen synthase kinase-3 (GSK-3)
Alternates names: HO:HO oxidoreductase
Taglines: A phosphorylating and an inactivating agent of glycogen synthase
NCBI Gene ID #: 847
NCBI Gene Symbol: CAT
Gene Source: Human
Accession #: P04040
Recombinant: Yes
Source: E. coli.
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): Activity of GSK-3 was determined by an in vitro kinase assay using 100 ng Tau as substrate and 1 µg of the recombinant GSK-3 in a 20 µl reaction containing 25 mM Tris-HCl, pH 7.5, 5 mM -glycerol phosphate, 12 mM MgCl, 2 mM DTT, 0.1 M Na3VO4, and 200 µM ATP. After 30 minutes incubation at 30°C, phosphorylation of Tau by the recombinant GSK-3 was determined by Western blot using a phospho-Tau antibody.
Biological activity:
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 244.0 kDa
Concentration: N/A
Appearance: Lyophilized powder
Physical form description: Lyophilized from 2 mg/ml in 0.1 M NH4CO3
Reconstitution Instructions: N/A
Amino acid sequence: N/A
