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Product Description
HAGH is a member of the glyoxalase family and a thiolesterase which hydrolyses S-lactoyl-glutathione to reduced glutathione and D-lactate. This protein is a detoxifying enzyme of glycolysis byproduct methylglyoxal and a target of p63 and p73 and serves as a pro-survival factor of the p53 family. It exists only as a monomer and binds two zinc ions per subunit. Recombinant human HAGH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Biovision | 7832 | HAGH human recombinant DataSheet
Biomolecule/Target: HAGH
Synonyms: Hydroxyacylglutathione hydrolase, GLO, GLX2, Glyoxalase II, HAGH1
Alternates names: Hydroxyacylglutathione hydrolase, GLO, GLX2, Glyoxalase II, HAGH1
Taglines: A thiolesterase which hydrolyses S-lactoyl-glutathione to reduced glutathione and D-lactate.
NCBI Gene ID #: 14256
NCBI Gene Symbol: Flt3-L
Gene Source: Human
Accession #: P49772
Recombinant: Yes
Source: E. coli.
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: Specific activity is > 1.8 unit/ml, in which one unit will hydrolyze 1.0 µmole of S-lactoylglutathione per minute at pH 7.5 at 25ºC.
Results: Specific activity is > 1.8 unit/ml
Binding Capacity: N/A
Unit Definition: One unit will hydrolyze 1.0 µmole of S-lactoylglutathione per minute at pH 7.5 at 25ºC.
Molecular Weight: 31.4 kDa (284 aa, 1-260 aa + His Tag)
Concentration: 0.5 mg/ml
Appearance: Liquid
Physical form description: 0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Reconstitution Instructions: N/A
Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MGSHMKVEVL PALTDNYMYL VIDDETKEAA IVDPVQPQKV VDAARKHGVK LTTVLTTHHH WDHAGGNEKL VKLESGLKVY GGDDRIGALT HKITHLSTLQ VGSLNVKCLA TPCHTSGHIC YFVSKPGGSE PPAVFTGDTL FVAGCGKFYE GTADEMCKAL LEVLGRLPPD TRVYCGHEYT INNLKFARHV EPGNAAIREK LAWAKEKYSI GEPTVPSTLA EEFTYNPFMR VREKTVQQHA GETDPVTTMR AVRREKDQFK MPRD
