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Product Description
Kallikrein-3 (KLK3) is also known as Prostate-specific antigen (PSA), APS, Gamma-seminoprotein (Seminin), P-30 antigen, SemenoGelase, is a glycoprotein enzyme, is a member of the kallikrein-related peptidase family and is secreted by the epithelial cells of the prostate gland. The preferential cleavage site of KLK3 is -Tyr-|-Xaa-, the catalytic activity is inhibited by SERPINA5 and is strongly inhibited by Zn2+. KLK3 hydrolyzes semenoGelin-1 thus leading to the liquefaction of the seminal coagulum.
Biovision | 7415 | Human CellExp Kallikrein-3 human recombinant DataSheet
Biomolecule/Target: Kallikrein-3
Synonyms: PSA, KLK3, KLKB1, Kallikrein-3, APS, KLK2A1, PSA, Hk3, Seminin, SemenoGelase
Alternates names: PSA, KLK3, KLKB1, Kallikrein-3, APS, KLK2A1, PSA, Hk3, Seminin, SemenoGelase
Taglines: Has important applications in the diagnosis of prostate cancer.
NCBI Gene ID #: UniGene:Rn.9869
NCBI Gene Symbol: IL1B
Gene Source: Human
Accession #: Q63264
Recombinant: Yes
Source: HEK293 cells
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: <1 EU/g by LAL method
Activity (Specifications/test method): N/A
Biological activity: Measured by its ability to cleave the colorimetric peptide substrate, Succinyl Arg Pro Tyr p Nitroanilide (Suc-RPY-pNA). The specific activity is >60 pmoles / min / g.
Results: Measured by its ability to cleave the colorimetric peptide substrate, Succinyl Arg Pro Tyr p Nitroanilide (Suc-RPY-pNA). The specific activity is >60 pmoles / min / g.
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 27.7 kDa. The predicted N-terminus is Ala 18. DTT-reduced Protein migrates as 36 kDa due to glycosylation.
Concentration: N/A
Appearance: Lyophilized
Physical form description: Lyophilized from 0.22 m filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Amino acid sequence: N/A
