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Product Description
Sonic hedgehog protein (SHH) also known as HHG-1, which belongs to the hedgehog family. SHH is expressed in fetal intestine, liver, lung, and kidney and not expressed in adult tissues. Sonic hedgehog protein can be cleavage into sonic hedgehog protein N-product and sonic hedgehog protein C-product by autolysis. SHH binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development. The C-terminal domain displays an auto-proteolysis activity and a cholesterol transferase activity. The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.
Biovision | 7223 | Human CellExp SHH human recombinant DataSheet
Biomolecule/Target: SHH
Synonyms: SHH, HHG-1, Sonic hedgehog, HLP3, HPE3, SMMCI, TPT, TPTPS
Alternates names: SHH, HHG-1, Sonic hedgehog, HLP3, HPE3, SMMCI, TPT, TPTPS
Taglines: Hedgehog signaling pathway ligand involved in regulating vertebrate organogenesis
NCBI Gene ID #: 3569
NCBI Gene Symbol: IL6
Gene Source: Human
Accession #: P05231
Recombinant: Yes
Source: HEK 293 cells
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).
Activity (Specifications/test method): Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 1.5-10 g/mL.
Biological activity: Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 1.5-10 g/mL.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: This protein is fused with 6 × His tag at the C-terminus, has a calculated MW of 20.4 kDa. The predicted N-terminus is Cys 24. DTT-reduced protein migrates as 22 kDa.
Concentration: N/A
Appearance: Lyophilized powder
Physical form description: Lyophilized from 0.22 m filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Amino acid sequence: N/A
