Product Description
Inosine monophosphate dehydrogenase, IMPDH, (E.C. 1.1.1.205) is the rate-limiting enzyme in de novo guanine nucleotide biosynthesis and it is essential for lymphocyte proliferation. IMPDH oxidizes inosine 5-monophosphate (IMP) to xanthine 5-monophosphate (XMP) using NAD as a cofactor. It plays a critical role in cell growth and in the malignancy of some tumors. IMPDH II is the predominant isoform of IMPDH and is specifically linked to a wide range of cancers and lymphocyte proliferation. BioVisions active human IMPDH II is suitable for functional assays, high-throughput screening and preclinical studies in drug discovery.
Biovision | P1131 | Human Inosine-5-Monophosphate Dehydrogenase II human recombinant DataSheet
Biomolecule/Target: IMPDH2
Synonyms: IMP dehydrogenase type II, IMPDH II, IMPD2, IMPDH
Alternates names: Peptidyl-prolyl cis-trans isomerase-like 2, CYC4, Cyp-60, CYP60, hCyP-60, GC33174.
Taglines: A protein that involved in RNA and/or DNA metabolism and regulation of cell growth
NCBI Gene ID #: 23759
NCBI Gene Symbol: PPIL2
Gene Source: Human
Accession #: Q13356
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: Specific activity is > 290 nmoles/min/mg
Results:
Binding Capacity: N/A
Unit Definition: One unit of enzyme converts 1 mole of IMP into XMP at 37ºC and pH 9
Molecular Weight: 61.6 kDa
Concentration: N/A
Appearance: Liquid
Physical form description: 20 mM Tris, pH 8.0, 150 mM NaCl, 2mM DTT, 2mM EDTA and 20% glycerol
Reconstitution Instructions: N/A
Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MGKRQHQKDK MYITCAEYTH FYGGKKPDLP QTNFRRLPFD HCSLSLQPFV YPVCTPDGIV FDLLNIVPWL KKYGTNPSNG EKLDGRSLIK LNFSKNSEGK YHCPVLFTVF TNNTHIVAVR TTGNVYAYEA VEQLNIKAKN FRDLLTDEPF SRQDIITLQD PTNLDKFNVS NFYHVKNNMK IIDPDEEKAK QDPSYYLKNT NAETRETLQE LYKEFKGDEI LAATMKAPEK KKVDKLNAAH YSTGKVSASF TSTAMVPETT HEAAAIDEDV LRYQFVKKKG YVRLHTNKGD LNLELHCDLT PKTCENFIRL CKKHYYDGTI FHRSIRNFVI QGGDPTGTGT GGESYWGKPF KDEFRPNLSH TGRGILSMAN SGPNSNRSQF FITFRSCAYL DKKHTIFGRV VGGFDVLTAM ENVESDPKTD RPKEEIRIDA TTVFVDPYEE ADAQIAQERK TQLKVAPETK VKSSQPQAGS QGPQTFRQGV GKYINPAATE QQRKSPQPVP LSPCPRRSPV GVLGTSAPGS SRLPDDH