Euro
USD
British Pound
NULL
Product Description
The protein coding region of the catalytic domain of PTP-1B (amino acids 1-321) was cloned into an E. coli expression vector. The catalytic domain of PTP-1B was overexpressed in E. coli as a soluble protein, and it was purified by conventional column chromatographic techniques.
Biovision | 6301 | Human Recombinant PTP1B DataSheet
Biomolecule/Target: N/A
Synonyms: Human Recombinant PTP1B
Alternates names: Protein tyrosine phosphatase, non-receptor type 1, PTPN1, Protein-tyrosine phosphatase 1B, Human Recombinant PTP1B,
Taglines: A negative regulator of the insulin signaling pathway
NCBI Gene ID #: 301291
NCBI Gene Symbol: IL-17F
Gene Source: Human
Accession #: Q5BJ95
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: 6,000-12,000 units/mg
Results: N/A
Binding Capacity: N/A
Unit Definition: Enzymatic activity is defined as the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37°C, pH 7.5 using 10 mM of substrate.
Molecular Weight: 37.3 kDa (321 amino acids)
Concentration: 1 mg/ml
Appearance: Liquid
Physical form description: 1 mg/ml solution in 25 mM Tris-HCl buffer (pH 7.5) containing 1 mM DTT, 2 mM mercaptoethanol, 1 mM EDTA, 20% Glycerol.
Reconstitution Instructions: N/A
Amino acid sequence: MEMEKEFEQI DKSGSWAAIY QDIRHEASDF PCRVAKLPKN KNRNRYRDVS PFDHSRIKLH QEDNDYINAS LIKMEEAQRS YILTQGPLPN TCGHFWEMVW EQKSRGVVML RVMEKGSLK CAQYWPQKEE KEMIFEDTNL KLTLISEDIK SYYTVRQLEL ENLTTQETRE ILHFHYTTWP DFGVPESPAS FLNFLFKVRE SGSLSPEHGP VVVHCSAGIG RSGTFCLADT CLLLMDKRKD PSSVDIKKVL LEMRKFRMGL IQTADQLRFS YLAVIEGAKF IMGDSSVQDQ WKELSHEDLE PPPEHIPPPP RPPKRILEPH N
