Product Description
Ubiquitin is a small polypeptide that can be conjugated via its C-terminus to amine groups of lysine residue on target proteins. This conjunction is referred to as monoubiquitylation. Additional ubiquitin moieties can be subsequently conjugated to this initial ubiquitin, utilizing any one of the seven lysine residues on the surface of ubiquitin. The formation of these ubiquitin chains is referred to as polyubiquitylation. This tag-free recombinant form of human ubiquitin is engineered to have all available lysines mutated to arginines, except at position 48. This molecule, therefore, can only form polyubiquitin chains of K48 linkage type. Covalent attachment of ubiquitin to other proteins serves various functions, but its major role is to target cellular proteins for destruction. Cellular components that activate, transfer, remove, or simply recognize ubiquitin number in the hundreds, perhaps even in the thousands. In light of this complexity the ubiquitin pathway is ideal for a systems biology approach. Ubiquitin plays a very important role in regulated non-lysosomal ATP dependent protein degradation. The Ub-proteasome proteolytic pathway, which is a complex process, is implicated to be of great importance for regulating numerous cellular processes.
Biovision | 6400 | Human Recombinant Ubiquitin-K48 DataSheet
Biomolecule/Target: N/A
Synonyms: Human Recombinant Ubiquitin-K48
Alternates names: Aldo-keto reductase family 1, member C 1, 20-alpha-HSD, DD1/DD2, HBAB, DDH, DDH1
Taglines: Serves as a substrate for K48 polyubiquitination
NCBI Gene ID #: 1645
NCBI Gene Symbol: AKR1C1
Gene Source: Human
Accession #: Q04828
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 90%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: Specific activity is approximately 0.15 0.2 units/mg.
Results:
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 38.9 kDa
Concentration: 4 mg/ml
Appearance: Liquid
Physical form description: 4 mg/ml in 50 mM Tris, pH 7.5, 150 mM NaCl, 10% glycerol.
Reconstitution Instructions: N/A
Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MDSKYQCVKL NDGHFMPVLG FGTYAPAEVP KSKALEATKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCNSH RPELVRPALE RSLKNLQLDY VDLYLIHFPV SVKPGEEVIP KDENGKILFD TVDLCATWEA VEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNQR KLLDFCKSKD IVLVAYSALG SHREEPWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTS EEMKAIDGLN RNVRYLTLDI FAGPPNYPFS DEY