Product Description
Ubiquitin C-terminal hydrolases (UCHs) are a family of cysteine hydrolases that catalyze the UBPs hydrolyze isopeptide bonds between ubiquitin and the substrate protein or within polyubiquitin chains. Human isopeptidase T (isoT) is an UBP capable of binding ubiquitin that specifically disassembles free polyubiquitin chains linked through isopeptide bonds involving the K48 of ubiquitin into ubiquitin monomers.
Biovision | 4861 | Isopeptidase T short form human recombinant DataSheet
Biomolecule/Target: N/A
Synonyms: USP5; Ubiquitin-specific protease 5
Alternates names: USP5; Ubiquitin-specific protease 5
Taglines: A ubiquitin-specific processing protease
NCBI Gene ID #: 20296
NCBI Gene Symbol: MCP-1
Gene Source: Human
Accession #: P10148
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: Determined by measuring the dose dependent chemotaxis with human THP-1 cells. The ED is in the range of 1-10 ng/ml
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 93.4 kDa
Concentration: 2.0 mg/ml
Appearance: Liquid
Physical form description: Supplied at a concentration of 2.0 mg/ml in 50 mM Tris, 10 mM -mercaptoethanol, pH 7.5.
Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1 mg/ml. This solution can then be diluted into other aqueous buffers or stored at 4°C for 1 week or 20°C for future use.
Amino acid sequence: N/A