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Product Description
Jacalin is isolated from Jackfruit seeds and purified by affinity chromatography. It is a glycoprotein with two non-covalently bound subunits (14.6 and 17.1 kDa) and belongs to the family of galactose-binding lectins. Jacalin is preferably used in applications to isolate IgA from human serum, isolating human plasma glycoproteins and for applications in histochemistry. BioVisions Jacalin is supplied as a white to light-yellow lyophilized powder without additives. The purity of the lectin is determined by SDS-PAGE, generating two homogeneous bands at 14 kDa and 17 kDa. Gene Bank Accession Number Q38720.
Biovision | 6560 | Jacalin DataSheet
Biomolecule/Target: N/A
Synonyms: Jacalin
Alternates names: Macrophage Colony Stimulating Factor, CSF-1, Lanimostim, MCSF, MGC31930, M-CSF.
Taglines: Galactose-binding lectin to purify IgA
NCBI Gene ID #: 1435
NCBI Gene Symbol: CSF1
Gene Source: Human
Accession #: P09603
Recombinant: No
Source: Human 293 cell expressed
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: < 0.1 EU/mg
Activity (Specifications/test method): N/A
Biological activity: The EC50 is typically 0.5 to 4 ng/mL for this protein with specific activity determined by the dose dependent stimulation of the proliferation of murine M-NFS-60 cells (Mouse Myeloid Leukemia indicator cell line)
Results: 0.5 to 4 ng/ml
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: This protein has 223 amino acids (Glu 33 - Arg 255) with a 6x-His tag at C-terminus, and a calculated MW of 26 kDa. The protein migrates to 35-45 kDa under reducing and non-reducing conditions (SDS-PAGE) due to glycosylation
Concentration: N/A
Appearance: Lyophilized
Physical form description: Lyophilized without additives
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS (pH 7.4). Do not vortex.
Amino acid sequence: N/A
