Product Description
Keratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with keratin 18. Keratin 8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. HepatoCellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18. The recombinant human keratin-8 is expressed in E. coli and purified by proprietary chromatographic techniques.
Biovision | 4817 | Keratin-8 human recombinant DataSheet
Biomolecule/Target: N/A
Synonyms: Keratin type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, KRT8, CYK8, KO, CK8, K2C8, CARD2,
Alternates names: Keratin type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, KRT8, CYK8, KO, CK8, K2C8, CARD2,
Taglines: Key structural material in skin, hair and nails
NCBI Gene ID #: 20296
NCBI Gene Symbol: MCP-1
Gene Source: Human
Accession #: P10148
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: 90%
Assay #2: HPLC
Endotoxin Level: <0.1 ng/g
Activity (Specifications/test method): N/A
Biological activity: The activity is determined by its ability to chemoattract human monocytes at 4 - 15 ng/ml.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 78.0 kDa
Concentration: 0.1 g/l
Appearance: Liquid
Physical form description: Sterile filtered (5 g/50 l) in Tris-Acetate (pH 7.5), 1 mM EDTA, 20% glycerol.
Reconstitution Instructions: Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Amino acid sequence: QPDAVNAPLT CCYSFTSKMI PMSRLESYKR ITSSRCPKEA VVFVTKLKRE VCADPKKEWV QTYIKNLDRN QMRSEPTTLF KTASALRSSA PLNVKLTRKS EANASTTFST TTSSTSVGVT SVTVN