Product Description
MART-1 (also known as Melan-A) is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Six other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, Tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. SubCellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. Human MART-1 is purified by proprietary chromatographic techniques.
Biovision | 4823 | MART-1/Melan-A human recombinant DataSheet
Biomolecule/Target: N/A
Synonyms: Melanoma antigen recognized by T-cells 1, Protein Melan-A, Antigen SK29-AA, Antigen LB39-AA
Alternates names: B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor.
Taglines: A melanocyte lineage marker
NCBI Gene ID #: 3586
NCBI Gene Symbol: IL10
Gene Source: Human
Accession #: P22301
Recombinant: Yes
Source: Human 293 cell expressed
Purity by SDS-PAGEs: > 95%
Assay: SDS-PAGE
Purity: 98%
Assay #2: HPLC
Endotoxin Level: < 1.0 EU per 1 g of protein
Activity (Specifications/test method): The specific activity was determined by the dose-dependent simulation of the proliferation of MC/9 cells (mouse mast cell line) in the presence of IL-4 using BioVisions Quick Cell Proliferation colorimetric Assay Kit Plus (Cat. No. K302). The ED50 is typically in a single digit ~ 1ng/ml.
Biological activity:
Results:
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 19 kDa with a 6×His tag at the C-terminus
Concentration: N/A
Appearance: Lyophilized protein
Physical form description: Sterile filtered and lyophilized with no additives
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS (pH 7.4). Do not vortex.
Amino acid sequence: Ser 19 Asn 178