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Product Description
Midkine and its functionally-related protein Pleiotrophin are heparin-binding neurotrophic factors that signal through the same receptor, known as anaplastic lymphoma kinase (ALK). MK plays an important regulatory role in epithelial-mesenchymal interactions during fetal development and in postnatal lung development. MK chemoattracts embryonic neurons, neutrophils and macrophages, and by signaling through the ALK receptor it exerts angiogenic, growth and survival activities during tumorigenesis. Recombinant murine Midkine is a 13.3 kDa protein containing 120 amino acid residues including five intra-molecular disulfide bonds.
Biovision | 7158 | Midkine murine recombinant DataSheet
Biomolecule/Target: Midkine
Synonyms: MK, NEGF-2
Alternates names: TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.
Taglines: Plays an important regulatory role in epithelial-mesenchymal interactions
NCBI Gene ID #: 7124
NCBI Gene Symbol: TNF
Gene Source: Human
Accession #: P01375
Recombinant: Yes
Source: Human 293 cell expressed
Purity by SDS-PAGEs: > 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: < 1.0 EU per 1 g of protein
Activity (Specifications/test method): N/A
Biological activity: ED is typically < 0.9 ng/mL. The specific activity was determined by the dose-dependent cytotoxity of the TNF alpha sensitive cell line L-929 in the presence of Actinomycin D.
Results: < 0.9 ng/ml
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 17 kDa, non-disulfide bonded homotrimer, glycosylated
Concentration: N/A
Appearance: Lyophilized powder
Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 0.4 x PBS, pH 7.4.
Reconstitution Instructions: Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.
Amino acid sequence: N/A
