Product Description
Dihydrofolate reductase (DHFR) is a member of the reductase family of enzymes that is ubiquitously expressed in all organisms. DHFR catalyzes the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate, and it is essential for the synthesis of thymidylate, purines and several amino acids. Expression of methotrexate (MTX)-resistant variants of DHFR in normal hematopoietic cells is a potential strategy to permit administration of larger doses of MTX by alleviating drug toxicity in normal cells and tissues that are drug sensitive.
Biovision | 6383 | Mouse Recombinant DHFR DataSheet
Biomolecule/Target: N/A
Synonyms: Mouse Recombinant DHFR
Alternates names: Dihydrofolate reductase.
Taglines: Reduces dihydrofolic acid to tetrahydrofolic acid
NCBI Gene ID #: 20302
NCBI Gene Symbol: CCL3
Gene Source: Mouse
Accession #: P10855
Recombinant: Yes
Source: E. Coli
Purity by SDS-PAGEs: 99%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: Determined by its ability to chemoattract Balb/c peripheral blood MNCs and mouse Balb/c splenocytes using a concentration range of 10-100 ng/ml.
Results: >0.2 units/mg
Binding Capacity: N/A
Unit Definition: One unit will convert 1.0 µmole of 7, 8 dihydrofolate and beta-NADPH to 5, 6, 7, 8-tetrahydrofolate and beta-NADP per minute at pH 6.5 at 25°C.
Molecular Weight: 23.8 kDa (207 aa, 1-187 aa + NT His-Tag)
Concentration: 1 mg/ml
Appearance: Liquid
Physical form description: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, 2 mM DTT and 10% glycerol.
Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1 mg/ml. This solution can then be diluted into other aqueous buffers
Amino acid sequence: N/A