Product Description
NFNB, also known as NFSB, shows the ability to reduce quinines. This protein is an enzyme for activating prodrugs in antibody directed enzyme prodrug therapy. It also capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5-(aziridin-1-yl)-2, 4-dinitrobenzamide). The reduction of CB1954 results in the generation of cytotoxic species. Recombinant E.coli NFNB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Biovision | 7830 | NFNB bacterial recombinant DataSheet
Biomolecule/Target: NFNB
Synonyms: Dihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive, dprA, nfsB, nfsI, ntr.
Alternates names: Dihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive, dprA, nfsB, nfsI, ntr.
Taglines: An enzyme for activating prodrugs in antibody directed enzyme prodrug therapy
NCBI Gene ID #: 6359
NCBI Gene Symbol: MIP5
Gene Source: E. Coli
Accession #: Q16663
Recombinant: Yes
Source: E. coli.
Purity by SDS-PAGEs: 97%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: Determined by its ability to chemoattract human T lymphocytes using a concentration range of 1.0-10 ng/ml.
Results: Specific activity: > 2 units/ml
Binding Capacity: N/A
Unit Definition: One unit will oxidize 1.0 µmole of b-NADH to b-NAD with 6.7-Dimethyldihydropterine (quinonoid isomer) as the non-nucleotide substrate per minute at pH 7.2 at 25ºC
Molecular Weight: 26.0 kDa (237 aa, 1-217 aa + His Tag)
Concentration: 1 mg/ml
Appearance: Liquid
Physical form description: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1 mM DTT and 50 mM NaCl
Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers
Amino acid sequence: N/A