Product Description
Activation of cell growth leads to the multiple phosphorylation of 40S ribosomal protein S6. The kinase responsible for controlling this event is termed p70S6K (1). Northern blot analysis shows p70S6K to be ubiquitously expressed in human adult tissues. p70S6K is activated by serum stimulation, and the serum-induced activation is inhibited by wortmannin and rapamycin. p70S6k activity undergoes changes in the cell cycle and increases 20-fold in G1 cells released from G0 (2). The kinase is reactivated 10-fold when cells released from a nocodazole-induced metaphase block enter G1 of the next cell cycle. The immunosuppressive agent rapamycin induces inactivation of p70s6k with no effect on other mitogen-activated kinases (3). The principal target of rapamycin-induced p70s6k inactivation is T389 site on p70S6K, which is located in an unusual hydrophobic sequence outside the catalytic domain. Mutation of T389 to alanine ablates kinase activity, whereas mutation to glutamic acid confers constitutive kinase activity and rapamycin resistance. p70S6K activation by growth factor requires phosphorylation by various inputs on multiple sites (4). p70S6K activation requires sequential phosphorylations at proline-directed residues in the putative autoinhibitory pseudosubstrate domain, as well as threonine 389. Threonine 229, a site in the catalytic loop is phosphorylated by phosphoinositide-dependent kinase 1 (PDK-1). Activation of p70S6K requires a phosphoinositide 3-kinase (PI3-K)-dependent signal(s).
Biovision | 7725 | p70 S6K Active DataSheet
Biomolecule/Target: N/A
Synonyms: p70, Ribosomal protein S6 kinase beta-1
Alternates names: GSK, Glycogen synthase kinase-3 (GSK-3)
Taglines: Increases protein synthesis and cell proliferation
NCBI Gene ID #: 2931
NCBI Gene Symbol: GSK3A
Gene Source: Human
Accession #: P49840
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: N/A
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): GSK-3 fusion protein at a concentration of 2 µg/20 µl reaction was phosphorylated in an in vitro kinase assay with 200 µM ATP. After 30 minutes incubation at 30°C, phosphorylation was detected by Western blot with phospho-specific GSK-3 antibodies.
Biological activity:
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 37.0 kDa
Concentration: 2 mg/ml
Appearance: Liquid
Physical form description: Lyophilized powder without additives
Reconstitution Instructions: N/A
Amino acid sequence: N/A