Product Description
Programmed cell death 1 ligand 2, also known as PDCD1LG2, is involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production. Recombinant human PDCD1LG2 protein, fused to His-tag at N-terminus, was expressed in E.coli.
Biovision | 7337 | PDCD1LG2 human recombinant DataSheet
Biomolecule/Target: PDCD1LG2
Synonyms: Programmed cell death 1 ligand 2, B7DC, bA574F11.2, Btdc, CD273, PD-L2, PDCD1L2, PDL2
Alternates names: Programmed cell death 1 ligand 2, B7DC, bA574F11.2, Btdc, CD273, PD-L2, PDCD1L2, PDL2
Taglines: Involved in the costimulatory signal essential for T-cell proliferation and IFNG production
NCBI Gene ID #: 4803
NCBI Gene Symbol: NGF
Gene Source: Human
Accession #: P01138
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: < 1.0 EU per 1 microgram of protein
Activity (Specifications/test method): N/A
Biological activity: N/A
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 22.8 kDa (204 aa, 20-200 aa + His Tag), confirmed by MALDI-TOF. (MW on SDS-PAGE shifts up)
Concentration: 1 mg/ml
Appearance: Liquid
Physical form description: 1 mg/ml in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, and 0.4 M Urea.
Reconstitution Instructions: Dissolve in 1x PBS (It is not recommended to reconstitute to a final concentration less than 100 g/ml.). This can further be diluted to other aqueous buffers.
Amino acid sequence: Recombinant human ProNGF produced in E. coli is a non-glycosylated, non-covalently linked homodimer with each polypeptide chain containing 222 amino acids with an extra N-terminal Met and having a molecular mass of 25 kDa. The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Pro-His-Ser.